The artificial intelligence industry entered 2025 with formidable momentum, building on a strong previous year. In 2024, 49 U.S. AI startups successfully raised funding rounds of $100 million or more, with three companies securing multiple "mega-rounds" and seven achieving rounds of $1 billion or larger.

This rapid pace of investment continued unabated into 2025. While fewer companies closed rounds exceeding $1 billion, a notable eight companies managed to raise multiple significant rounds, a substantial increase compared to 2024. The outlook for 2026 also appears promising, with early announcements like Elon Musk's xAI securing a $20 billion Series E round and Sam Altman's brain-computer interface startup, Merge Labs, raising a $250 million seed round (led by OpenAI) within the first few weeks of the year. While it's still early, these indicators suggest sustained growth in the AI sector.

Below is a comprehensive list of U.S. AI companies that raised $100 million or more in 2025:

December Funding Highlights

  • Austin, Texas-based Mythic, specializing in power-efficient compute for AI, closed a $125 million venture round on December 17. The round was led by DCVC, with participation from SoftBank, NEA, and Linse Capital.
  • Chai Discovery, which develops AI models for biotech and drug discovery, announced a $130 million Series B round on December 15, valuing the company at $1.2 billion. Oak HC/FT and General Catalyst co-led the investment.
  • Generative media platform Fal secured its third funding round of 2025 on December 9. This $140 million Series D round, led by Sequoia, tripled the company's valuation to over $4.5 billion.
  • Unconventional AI, a one-year-old startup rethinking the foundation of computers for the AI age, confirmed a massive $475 million seed round on December 8. The round, led by Lightspeed Venture Partners and Andreessen Horowitz, valued the company at nearly $4.5 billion.
  • Boston-based 7AI, a developer of cybersecurity AI agents, announced a $130 million Series A round on December 4. Index Ventures led the round, with participation from Greylock, Spark Capital, and CRV.

November Funding Highlights

  • All-in-one AI workspace platform Genspark announced a $275 million Series B round on November 20, valuing the company at $1.25 billion. Investors included Emergence Capital Partners, SBI Investment, and LG Technology Ventures.
  • Luma AI, known for its photo and video creation models, raised $900 million in a Series C round, valuing the startup at $4 billion. Humain led the round, with Andreessen Horowitz, AMD Ventures, and Amplify Partners participating.
  • Anysphere, creator of the viral vibe-coding platform Cursor, secured $2.3 billion in a funding round announced on November 13, valuing the company at $29.3 billion. This was the company's second funding round of the year.
  • Parallel, building web infrastructure for AI agents, announced a $100 million Series A round on November 12, co-led by Index Ventures and Kleiner Perkins.
  • Healthcare AI agent startup Hippocratic AI raised a $126 million Series C round on November 3, valuing the company at $3.5 billion. Led by Avenir Growth, this was the company's second round of the year.

October Funding Highlights

  • Fireworks AI, a platform for building AI applications using open-source models, announced a $250 million Series C round on October 28, valuing the company at $4 billion.
  • Enterprise AI startup Uniphore achieved a $2.5 billion valuation after a $260 million Series F round announced on October 22. Investors included Snowflake Ventures, Nvidia, Databricks Ventures, and AMD.
  • Sesame, a voice AI company, raised a $250 million Series B round on October 21, co-led by Sequoia and Spark Capital, with participation from SignalRank.
  • Cambridge, Massachusetts-based OpenEvidence, which builds an AI chatbot for the medical field, secured its second funding round of 2025. The $200 million Series C round, announced on October 20, valued the company at $6 billion.
  • Lila Sciences, aiming to build a science superintelligence platform, announced its second funding round of 2025 on October 14. The $350 million Series A round was co-led by Braidwell and Collective Global.
  • DeepSeek competitor Reflection AI announced its second mega-round of the year on October 9. The $2 billion Series B round, led by Nvidia, valued the company at $8 billion.
  • EvenUp, which develops AI for the personal injury legal field, announced a $150 million Series E round on October 7, valuing the company at over $2 billion. Bessemer led the round, with Lightspeed, Bain Capital, and SignalFire participating.

September Funding Highlights

  • Periodic Labs, focused on building an AI scientist, announced a $300 million seed round on September 30. Felicis and Andreessen Horowitz led the round, with Nvidia, Lightspeed, and Khosla Ventures participating.
  • AI infrastructure company Cerebras Systems raised a substantial $1.1 billion Series G round on September 30, valuing the company at $8.1 billion. Fidelity and Atreides Management co-led the round.
  • Modular announced a $250 million funding round on September 24. The round was led by US Innovative Technology Fund, with participation from GV, Greylock, and General Catalyst.
  • Distyl AI, which develops AI enterprise software, raised a $175 million Series B round announced on September 23. This round valued the startup at $1.8 billion and included investors like Khosla Ventures and Lightspeed.
  • AI infrastructure startup Upscale AI secured a sizable $100 million seed round on September 17, co-led by Maverick Silicon and Mayfield. StepStone Group, Stanford University, and Qualcomm Ventures also participated.
  • Groq, an AI inference company, raised a $750 million Series D-3 round on September 17, valuing the company at nearly $6.9 billion. The round was led by Disruptive.
  • AI training startup Invisible Technologies was valued at $2 billion after a $100 million fundraise announced on September 16. Vanara Capital led the raise, with Greycroft, Tallwoods Capital, and Freestyle Capital participating.
  • Cognition AI, creator of the vibe-coding agent Devin, raised a $400 million Series C round announced on September 8. Founders Fund led the round, valuing the company at $10.2 billion.
  • AI Infrastructure startup Baseten raised a $150 million Series D round on September 5, valuing the company at $2.1 billion. Bond led the round, with CapitalG, IVP, and Spark Capital participating.
  • Bret Taylor's customer service AI agent platform Sierra raised $350 million in a round led by Greenoaks Capital, announced on September 4. This fundraise valued Sierra at over $10 billion.
  • You.com, a personalized AI search engine, raised a $100 million Series C round led by Cox Enterprises, announced on September 3. The company was valued at $1.5 billion.
  • AI research lab Anthropic raised its second round of 2025 in September. On September 2, Anthropic announced a $13 billion Series F round, valuing the company at $183 billion. Iconiq, Fidelity, and Lightspeed led the round.

August Funding Highlights

  • Healthcare and housing automation platform EliseAI raised $250 million in a Series E round announced on August 20, valuing the startup at $2.2 billion. Andreessen Horowitz led the round.
  • Decart, an AI research lab, raised $100 million at a $3.1 billion valuation. The round, announced on August 7, included Sequoia Capital, Benchmark, and Zeev Ventures.

July Funding Highlights

  • Generative media platform Fal raised a $125 million Series C round led by Meritech Capital Partners. The company announced the round, which values Fal at $1.5 billion, on July 31. Salesforce Ventures, Shopify Ventures, and Google AI Futures Fund also joined.
  • Five-year-old Ambience Healthcare, building an AI healthcare operating system, raised a $243 million Series C round, co-led by Oak HC/FT and Andreessen Horowitz. Kleiner Perkins, OpenAI Startup Fund, and Smash Capital also participated.
  • Reka AI, an AI research lab, raised $110 million in a Series B round announced on July 22, valuing the company at $1 billion. Snowflake and Nvidia were among the investors.
  • AI research lab Thinking Machines Lab confirmed it raised $2 billion on July 15. This sizable seed round, led by Andreessen Horowitz, with Nvidia, Accel, and AMD participating, valued the company at $12 billion.
  • Cambridge, Massachusetts-based OpenEvidence, developing an AI-powered search tool for clinicians, raised $210 million at a $3.5 billion valuation. The Series B round, announced on July 15, was led by Kleiner Perkins and GV.
  • Harmonic, focused on building a mathematical reasoning engine, raised a $100 million Series B round led by Kleiner Perkins. The round, announced on July 10, valued the company at $875 million.

June Funding Highlights

  • Healthcare AI unicorn Abridge announced a $300 million Series E round, valuing the company at $5.3 billion. Led by Andreessen Horowitz with Khosla Ventures participating, this was Abridge's second round of 2025.
  • Harvey, which builds AI tools for the legal industry, announced its second $300 million round of 2025 on June 23. This latest Series E round, co-led by Kleiner Perkins and Coatue, brought the company's valuation to $5 billion.
  • Healthcare AI startup Tennr announced a $101 million Series C round led by IVP, with participation from Lightspeed Venture Partners, GV, and Andreessen Horowitz. The round valued the company at $605 million.
  • Enterprise search startup Glean announced a $150 million Series F round on June 10, led by Wellington Management, with Sequoia, Lightspeed Venture Partners, and Kleiner Perkins participating. Glean is now valued at $7.25 billion.
  • Anysphere, the AI research lab behind AI coding tool Cursor, raised a sizable $900 million Series C round, valuing the company at nearly $10 billion. Thrive Capital led the round, with Andreessen Horowitz, Accel, and DST Global participating.

May Funding Highlights

  • AI data labeling startup Snorkel AI announced a $100 million Series D round on May 29, valuing the company at $1.3 billion. Addition led the round, with Prosperity7 Ventures, Lightspeed Venture Partners, and Greylock participating.
  • LMArena, a popular community-driven benchmarking tool for AI models, raised a $100 million seed round announced on May 21, valuing the startup at $600 million. Andreessen Horowitz and UC Investments co-led the round.
  • Las Vegas-based AI infrastructure company TensorWave announced a $100 million Series A round on May 14. The round was co-led by Magnetar Capital and AMD Ventures.

April Funding Highlights

  • SandboxAQ closed a $450 million Series E round on April 4, valuing the AI model company at $5.7 billion. Investors included Nvidia, Google, and Bridgewater Associates founder Ray Dalio.
  • Runway, which creates AI models for media production, raised a $308 million Series D round announced on April 3, valuing the company at $3 billion. General Atlantic led the round, with SoftBank, Nvidia, and Fidelity participating.

March Funding Highlights

  • AI behemoth OpenAI raised a record-breaking $40 billion funding round that closed on March 31, valuing the startup at $300 billion. SoftBank led the round, with Thrive Capital, Microsoft, and Coatue participating.
  • On March 25, Nexthop AI, an AI infrastructure company, announced a Series A round of $110 million led by Lightspeed Venture Partners. Kleiner Perkins, Battery Ventures, and Emergent Ventures also participated.
  • Cambridge, Massachusetts-based Insilico Medicine

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