The U.S. artificial intelligence sector is witnessing an unprecedented surge in venture funding in 2025, already eclipsing the monumental investment levels of the previous year. To date, over 50 American AI startups have successfully secured funding rounds of $100 million or more, with a notable trend of multiple companies closing successive mega-rounds. This robust influx of capital underscores sustained investor confidence and the rapid expansion of AI innovation across diverse applications.

Last year, 2024, saw 49 U.S. startups raise $100 million or more, including seven companies that secured $1 billion-plus rounds. This year, 2025, has already matched that pace in terms of the number of companies reaching the $100 million threshold, and significantly more companies have raised multiple funding rounds exceeding this amount. The following list details the U.S. AI companies that have raised $100 million or more this year, organized by the month of their announcement:

November

  • Anysphere, the maker of the AI coding platform Cursor, raised $2.3 billion in a funding round announced on November 13. This round valued the company at $29.3 billion and marked its second major funding event of the year.
  • Parallel, which develops web infrastructure for AI agents, announced a $100 million Series A round on November 12. The round was co-led by Index Ventures and Kleiner Perkins.
  • Healthcare AI agent startup Hippocratic AI closed a $126 million Series C round, valuing the company at $3.5 billion. Announced on November 3, this was the company's second funding round this year and was led by Avenir Growth.

October

  • Fireworks AI, a platform enabling users to build AI applications with open-source models, announced a $250 million Series C round on October 28, valuing the company at $4 billion.
  • Enterprise AI startup Uniphore achieved a $2.5 billion valuation after a $260 million Series F round announced on October 22. Investors included Snowflake Ventures, Nvidia, Databricks Ventures, and AMD.
  • Sesame, a voice AI company, raised a $250 million Series B round co-led by Sequoia and Spark Capital. The round, announced on October 21, also saw participation from SignalRank.
  • Cambridge, Massachusetts-based OpenEvidence, which builds an AI chatbot for the medical field, secured its second funding round of 2025. The $200 million Series C round was announced on October 20 and valued the company at $6 billion.
  • Lila Sciences, focused on building a science superintelligence platform, announced its second funding round of 2025 on October 14. The $350 million Series A round was co-led by Braidwell and Collective Global.
  • DeepSeek competitor Reflection AI announced its second mega-round of the year on October 9. The $2 billion Series B round, led by Nvidia, valued the company at $8 billion.
  • EvenUp, which develops AI for the personal injury legal field, announced a $150 million Series E round on October 7, valuing the company at over $2 billion. The round was led by Bessemer, with participation from Lightspeed, Bain Capital, and SignalFire.

September

  • Periodic Labs, building an AI scientist, announced a $300 million seed round on September 30. Felicis and Andreessen Horowitz led the round, with Nvidia, Lightspeed, and Khosla Ventures participating.
  • Cerebras Systems, an AI infrastructure company, raised a substantial $1.1 billion Series G round, valuing the company at $8.1 billion. The round, announced on September 30, was co-led by Fidelity and Atreides Management.
  • Modular announced a $250 million funding round on September 24. The round was led by the US Innovative Technology Fund, with participation from GV, Greylock, and General Catalyst.
  • Distyl AI, a developer of AI enterprise software, raised a $175 million Series B round, announced on September 23. This round valued the startup at $1.8 billion and included investors like Khosla Ventures and Lightspeed.
  • AI infrastructure startup Upscale AI secured a significant $100 million seed round, co-led by Maverick Silicon and Mayfield. Announced on September 17, the round also included StepStone Group, Stanford University, and Qualcomm Ventures.
  • Groq, an AI inference company, raised a $750 million Series E round, valuing the company at nearly $6.9 billion. The round was announced on September 17 and led by Disruptive.
  • AI training startup Invisible Technologies was valued at $2 billion after a $100 million fundraise announced on September 16. The raise was led by Vanara Capital, with participation from Greycroft, Tallwoods Capital, and Freestyle Capital.
  • Cognition AI, the creator of the AI coding agent Devin, raised a $400 million Series C round, announced on September 8. The round was led by Founders Fund and valued the company at $10.2 billion.
  • AI Infrastructure startup Baseten raised a $150 million Series D round, valuing the company at $2.1 billion. The September 5 round was led by Bond, with participation from CapitalG, IVP, and Spark Capital.
  • Bret Taylor’s customer service AI agent platform Sierra raised $350 million in a round led by Greenoaks Capital. This fundraise, announced on September 4, valued Sierra at over $10 billion.
  • You.com, a personalized AI search engine, raised a $100 million Series C round led by Cox Enterprises. The round was announced on September 3 and valued the company at $1.5 billion.
  • AI research lab Anthropic raised its second round of 2025 in September. Anthropic announced a $13 billion Series F round on September 2, valuing the company at $183 billion. The round was led by Iconiq, Fidelity, and Lightspeed.

August

  • Healthcare and housing automation platform EliseAI raised $250 million in a Series E round that valued the startup at $2.2 billion. The round, announced on August 20, was led by Andreessen Horowitz.
  • Decart, an AI research lab, raised $100 million at a $3.1 billion valuation. The round, announced on August 7, included Sequoia Capital, Benchmark, and Zeev Ventures.

July

  • Generative media platform Fal raised a $125 million Series C round led by Meritech Capital Partners. The company announced the round, which values Fal at $1.5 billion, on July 31. Salesforce Ventures, Shopify Ventures, and Google AI Futures Fund also joined.
  • Five-year-old Ambience Healthcare, building an AI healthcare operating system, raised a $243 million Series C round led by Oak HC/FT and Andreessen Horowitz. Kleiner Perkins, OpenAI Startup Fund, and Smash Capital also participated.
  • Reka AI, an AI research lab, raised $110 million in a Series B round that included Snowflake and Nvidia. Announced on July 22, the round values the company at $1 billion.
  • AI research lab Thinking Machines Lab confirmed it raised $2 billion on July 15. This substantial seed round, led by Andreessen Horowitz with participation from Nvidia, Accel, and AMD, valued the company at $12 billion.
  • Cambridge, Massachusetts-based OpenEvidence, developing an AI-powered search tool for clinicians, raised $210 million at a $3.5 billion valuation. The Series B round, announced on July 15, was led by Kleiner Perkins and GV.
  • Harmonic, which is building a mathematical reasoning engine, raised a $100 million Series B round led by Kleiner Perkins. The round was announced on July 10 and values the company at $875 million.

June

  • Healthcare AI unicorn Abridge announced it raised a $300 million Series E round that values the company at $5.3 billion. The round, led by Andreessen Horowitz with Khosla Ventures participating, was the company’s second round of 2025.
  • Harvey, which builds AI tools for the legal industry, announced it raised its second $300 million round of 2025 on June 23. This latest Series E round was co-led by Kleiner Perkins and Coatue, bringing the company’s valuation to $5 billion.
  • Healthcare AI startup Tennr announced it raised a $101 million Series C round led by IVP, with participation from Lightspeed Venture Partners, GV, and Andreessen Horowitz. The round values the company at $605 million.
  • Enterprise search startup Glean announced a $150 million Series F round on June 10, led by Wellington Management, with participation from Sequoia, Lightspeed Venture Partners, and Kleiner Perkins. Glean is now valued at $7.25 billion.
  • Anysphere, the AI research lab behind the AI coding tool Cursor, raised a substantial $900 million Series C round that values the company at nearly $10 billion. The round was led by Thrive Capital, with participation from Andreessen Horowitz, Accel, and DST Global.

May

  • AI data labeling startup Snorkel AI announced a $100 million Series D round on May 29, valuing the company at $1.3 billion. The round was led by Addition, with participation from Prosperity7 Ventures, Lightspeed Venture Partners, and Greylock.
  • LMArena, a popular, community-driven benchmarking tool for AI models, raised a $100 million seed round that valued the startup at $600 million. The round, announced on May 21, was co-led by Andreessen Horowitz and UC Investments, with Lightspeed Venture Partners, Kleiner Perkins, and Felicis also participating.
  • Las Vegas-based AI infrastructure company TensorWave announced a $100 million Series A round on May 14. The round was co-led by Magnetar Capital and AMD Ventures, with participation from Prosperity7 Ventures, Nexus Venture Partners, and Maverick Silicon.

April

  • SandboxAQ closed a $450 million Series E round on April 4, valuing the AI model company at $5.7 billion. The round included Nvidia, Google, and Bridgewater Associates founder Ray Dalio among other investors.
  • Runway, which creates AI models for media production, raised a

    Similar News